Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 18 - Jul 23
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human SEMA3B ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a semaphorin-3B (SEMA3B) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of semaphorin-3B (SEMA3B) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Semaphorin-3B ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Semaphorin-3B is a protein encoded by the SEMA3B gene. The semaphorin/collapsin family of molecules plays a key role in guiding growth cones during neuronal development. Secreted proteins encoded by members of this gene family are important in axon guidance and have been shown to function as tumor suppressors by inducing apoptosis. Diseases associated with SEMA3B include phenylcyclic drug abuse and lung cancer. Its relevance also includes neuroscience. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.8 ★★★★★
Based on 24 reviews
Sort
Product Reviews
★★★★★ 5
Budget-Friendly with a Rustic Charm - WOPITUES Wood Floating Shelves Set of 6
Size: 16.5 Inch, Color: Rustic Brown
The WOPITUES Wood Floating Shelves Set of 6 is an appealing option for those looking to add a touch of rustic charm to their home without spending a fortune. This set of six shelves offers great versatility and functionality, ideal for displaying a variety of items such as books, plants, and decorative pieces, enhancing any wall decor with a farmhouse vibe.
Upon installation, which is straightforward with the included mounting hardware, these shelves provide a neat and organized look. The ability to arrange them in various configurations allows for creative freedom in decorating, making it easy to personalize your space. The overall capacity and sturdiness of the shelves are commendable, holding up well under the weight of typical decor items.
However, while the shelves perform well from a distance, a closer inspection reveals that the materials look somewhat cheap. The wood finish, although attractive from afar, does not hold up to close scrutiny, with the texture and detailing lacking the quality one might expect from more expensive shelving. This aspect might be a downside for those who prefer more authentic, high-quality woodwork.
In conclusion, the WOPITUES Wood Floating Shelves are a solid choice for anyone on a budget looking to enhance their home decor. They offer good functionality and aesthetic appeal at a distance, though they may not satisfy those looking for premium material quality. For the price, they are a worthy consideration for casual use and decorative purposes.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 7, 2024
★★★★★ 4
Good product. Real wood. Sturdy wall anchor.
Size: 16.5 Inch, Color: Black
4.5 stars. It's a great product. The pictures made me wonder if this was wood or plastic, but it is definitely wood. It is very attractive and fairly easy to assemble. I feel like the harnesses for the wall don't fit the wood well enough, so you can get different distances from the wall, and one of my shelves is half a centimeter below the others, even though I lined it up the same. But they are sturdy shelves and very pretty.
My only real complaint is the screw anchors for the walls. They are SERIOUS. I doubt these will ever fall out of the wall, for real. The only problem is, they eat the wall as you are putting them in. If I ever decide to move these, I will have to do some serious dry wall repair.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 4, 2026
★★★★★ 5
Easy to install
Size: 16.5 Inch, Color: White
I bought these for my step daughter for her birthday. I installed them on about 45 minutes. Theyre small and decorative. They're nice to store light weight knick knacks on.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 16, 2026
★★★★★ 5
Simple, sturdy, and looks great in the room
Size: 16.5 Inch, Color: Rustic Brown
I bought this farmhouse shelf for our bedroom because I needed extra storage that would not make the space feel cramped. After using it for a few weeks, I can say it fits the room really well and does exactly what I hoped it would.
The rustic brown color is warm without looking orange or overly dark. It has that farmhouse style but still feels clean and modern. It blends nicely with neutral furniture and does not look cheap or overly distressed.
Assembly took me about thirty minutes. The instructions were easy to follow and all the hardware was included. Once everything was tightened, the shelf felt stable and did not wobble. I made sure to assemble it on a flat surface and tighten the screws evenly, which helped keep it level.
Right now I am using it for books, a small plant, framed photos, and a storage basket. It handles normal household items without any sagging. I would not overload it with very heavy textbooks on every shelf, but for everyday use in a bedroom or living room, it feels solid.
One thing I really like is the size. It gives you good vertical storage without taking up too much floor space. It works especially well in smaller rooms or apartments where you want storage but do not want bulky furniture.
If using it in a bathroom, I would just be mindful of humidity over time since the shelves appear to be engineered wood with a laminate finish. For towels and lightweight storage bins, it should work well.
Overall, this is a practical and good looking shelf. It is not high end furniture, but it does not feel flimsy either. For the price, I would buy it again.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 25, 2026
★★★★★ 5
Great for the price you pay
Size: 16.5 Inch, Color: Rustic Brown, Size: 16.5 Inch, Color: Rustic Brown
Great shelves for the price you pay. They look good and seem to have good stability so far; we’ll see if anything falls on heads. I put a 6 pack of each up in my son’s rooms and they have all kinds of stuff from katanas to books to knick knacks on them. The shelves aren’t finished so they can leave slivers and might be harder to clean with just a cloth. They came with 6 pieces of wood, not pre-drilled or with any marks for holes, a pack of pretty tough brackets, and all mounting hardware. We used a level, hammer, stud finder, tape measure, the provided wall anchors, long and short screws, pencil, 13/64 drill bit, Phillips head bit, and drill. These helped save space and get rid of some of their clutter.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 10, 2026
recommand products
Gulfport, Beach Party, White, Men's Short Sleeve Round Neck T-shirt 00279
20.00
Dibut Bay, Beach Party, White, Men's Short Sleeve Round Neck T-shirt 00279
20.00
Hartigan, Beach Party, White, Men's Short Sleeve Round Neck T-shirt 00279
20.00
York Devils Mercer+Mettle Quilted Full-Zip Jacket
75.00
Auroville, Beach Party, White, Men's Short Sleeve Round Neck T-shirt 00279
20.00