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Description
Mouse DRD1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000 × g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with ice-cold PBS and resuspend them in 150-200 μL of PBS per 1 × 10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500 × g for 10 minutes at 2-8°C, and collect the supernatant for analysis. 5. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a dopamine receptor D1 (DRD1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the dopamine receptor D1 (DRD1) content in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Dopamine Receptor D1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Dopamine receptor D1 (DRD1) is a protein that is one of two types of the D1 receptor family—receptors D1 and D5—encoded by the DRD1 gene. It is the most abundant dopamine receptor in the central nervous system. The D1 receptor shares a high degree of structural homology with another dopamine receptor, D5, and they bind similar drugs. Therefore, none of the known orthotropic ligands are selective for D1 versus D5 receptors. However, benzodiazepines generally exhibit greater selectivity for D1 and D5 receptors over D2 receptors. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
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4.1 ★★★★★
Based on 19 reviews
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Product Reviews
★★★★★ 5
Very nice pillow
Color: Cut-out White, Size: King
I was skeptical of spending this much money on a pillow, but so far I'm glad I did.
It's very comfortable, and the shape does seem to help. I injured my neck/shoulder years ago, and I would wake up 1-2x per week heading slept wrong making my neck and shoulder stiff and painful in the morning. This pillow has so far prevented that.
This pillow has also significantly reduced my snoring, according to both my Samsung watch and my wife.
I'm a side sleeper.
This pillow can be as firm or soft as you want since you can add or remove filling. It comes with a bag of extra filling just for that.
I wouldn't say its cooling, but it certainly isnt too warm either. And I'm someone who sleeps hot.
All in all, I'd buy it again.
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Reviewed in the United States on May 28, 2026
★★★★★ 5
this is it (hopefully), no chemical odor
Color: Original White, Size: Queen
Over the years, I have spent a lot of time and money buying pillows. I can never seem to find one that fits my needs. I thought this would be a good option to try because this came up as a top rated pillow from consumer reports.
I really think this pillow might be the last pillow I ever need. I saw all the good reviews and I also read all the bad reviews. Pillows are very personal, and a pillow that works for one person may not work for the other person but, I decided to give this a try.
I was so excited. This was on sale at Amazon and I got it same day. I have a lot of neck issues and pinched nerves so I thought this pillow would help. Once I took it out of the box, I fluffed it up by hand because I don’t have a dryer. Within 15 minutes the pillow was really fluffy. I tested it out. I’m a side sleeper. It didn’t work too well. I removed some of the stuffing which is luxuriously soft. And it seemed to be comfortable. This morning I woke up with a horrible neck and headache. So, I took a little more stuffing out. It seems to feel better now. This gives just the right amount of support. It doesn’t give too much pushback and it also doesn’t go flat. Yes, this will flatten out a little bit when you lay your head on it, but not completely to the mattress flat. I fluff it up before I go to bed and it works great. I think I have it at just the right thickness but we will see how it goes. Surprisingly, it was not too hot for me like others have reported. I do have a temperature control system on the bed so maybe that’s why the pillow temperature didn’t bother me as much. I did not have any pain in my ears from sleeping on a hard pillow. That was a big plus. It gives Justin enough support and not too much pressure so it’s perfect. The outer shell is really nice. The shell and zippers seem to be good quality. I still use a genuine silk pillowcase because I like it. I think this is my forever pillow. I will add an update after I use it for a while if anything changes. I am hoping for good durability. Last thing, this also comes with extra stuffing if you like your pillow, extra high, or if your pillow starts to go flat, you have more stuffing to put in it. Putting your pillow out in the sun or in the dryer once every couple of months will help to keep it fluffy. I don’t think I would ever wash the foam on the inside. There’s no reason for that. The outer shell is what’s going to get most dirty.
Just know, I liked it so much I bought another one immediately.
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Reviewed in the United States on November 21, 2023
★★★★★ 4
Rediscoving the Great Qualities of this Pillow (after Originally Rejecting It)
Color: Original White, Size: Queen
I recently 'rediscovered' this pillow... having purchased it a year or so ago, when neck + shoulder issues prevented me from being very happy with *ANY* pillow! Believe me, I tried so many! Back then, after repeatedly adjusting the fill of this COOP queen-size pillow, I ultimately stashed the whole thing in a closet. But now, it has unexpectedly re-entered my life... and I'm finally liking it.
It helps that I've resolved most of the neck + shoulder issues (thanks to a year of PT). Plus. I realized that I had removed way too much fill... leaving about as much support as a pile of gently scrambled eggs. So, now that I'm in much better condition and have added back a lot of fill, I'm finding this pillow quite satisfying... yes, actually good! These days, I wake up each morning with a much happier neck.
I do not. however, use the thick, quilted, zippered outer cover that came with it... as I find that stiffens + firms up the pillow a little too much for my taste. So, on top of the thinner, stretchy, zippered inner cover (also provided with the pillow, to contain the fill,) I've replaced the quilted outer cover with a jersey-stretch, zippered, cotton pillow protector. which is lighter weight and more malleable.
As a person who has been traveling for years—by necessity—with a personal pillow from home, I'm now recognizing the extra advantages of this COOP pillow: For travel, its parts all compress well + easily (while for sleeping, the fill is light + lofty, but also supportive, as long as you don't remove way too much). And the pillow's components all readily come apart, so they can be packed separately...even the stuffing can be separated into smaller zip-lock packets, if needed—meaning there's never a need to squish an entire, impossibly big, somewhat dense pillow into a suitcase (or even a compression sack). Quick + easy to disassemble and reassemble. Really, the COOP can be treated as a build-your-own pillow (remember, I changed out the zippered outer cover and adjusted the stuffing volume to my own prefences). It's not cheap, but these days, remarkably, it's doing the trick for me. So glad I didn't get rid of it.
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Reviewed in the United States on February 27, 2026
★★★★★ 5
Great pillow and company
Color: Crescent White, Size: King
I have a couple of Coop Home pillows and loved them. They are several years old and I was on the search for a replacement. I tried 2 other brands, one cost more and the other cost less. Returned 1 and used the other for a short time before I had to go back to my old Coop pillow. I started looking at crescent pillows and did some research. This type of pillow is great for side and back sleepers. Being a side sleeper I looked into the best rated. Coop Home crescent pillow was not rated #1 but it was close. The top rated pillow cost 2.5 times more and I could not justify paying that. I am happy to report that I am waking up less (before every 1.5-2 hours). It comes with extra fill and I love a very firm pillow so I emailed customer service to see if I could get more filling. This was on the weekend and in 1 day they responded that they would be happy to. Now that is great customer service. I sleep warm and have no problem with a hot head. It comes with a cover but I use silk pillowcases and it works fine. I will say when I first got the pillow it was very thin and flimsy. I had some doubts but followed directions and put in the dryer on low. Came out perfect. Highly recommend this pillow and company.
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Reviewed in the United States on April 20, 2026
★★★★★ 5
Best pillow I've ever owned
Color: Cut-out White, Size: Queen
While the pillow looks anemic when it first comes out of its packaging, it puffs/firms up beautifully after time in the dryer. I haven't had any odor issues.
I'm mostly a side sleeper and at first I thought there wasn't enough of the extra fill, so I put it all in. I've ended up needing to take at least half back out to keep my head at the correct angle.
While this can fit in a standard pillow case, it just barely fits and keeps coming out - at least at my fill level. Queen pillow cases are much better.
This is so much more comfortable than any of my previous pillows. I don't need to stack multiple pillows to get the right support. I'm no longer waking up with neck or shoulder or arm pain. I'm recommending it to pretty much everyone and I've purchased a second pillow to keep at my parents for when I visit!
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Reviewed in the United States on May 6, 2026