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Description
Human ARNTL ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Aryl hydrocarbon receptor nuclear translocator-like protein 1 | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Aryl hydrocarbon receptor nuclear translocator-like protein, also known as ARNTL or brain and muscle ARNT-like protein 1 (BMAL1), is a protein encoded by the BMAL1 gene. BMAL1 encodes a transcription factor with a basic helix-loop-helix (bHLH) structure and two PAS domains. The human Arntl gene has 24 exons and is located on band p15 of chromosome 11. This 62-amino acid protein plays a key role as a positive element in the mammalian autoregulatory transcription-translation negative feedback loop (TTFL), which is responsible for generating molecular circadian rhythms. Research has shown that it is the only clock gene without which the biological clock cannot function in humans. It has also been identified as a candidate gene for susceptibility to hypertension, diabetes, and obesity, and its mutations have been associated with infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Genome-wide analyses have estimated that it targets over 150 sites in the human genome, including all clock genes and genes encoding proteins that regulate metabolism. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.1 ★★★★★
Based on 619 reviews
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Product Reviews
★★★★★ 5
Phenomenal book, one major criticism
Format: Kindle
Great book. Dalio does a remarkable job seeing the bigger picture and providing confidence through historical events/ever repeating cycles that you can predict at a high level what is coming next for a given country or the world and plan accordingly. The corkscrew of evolution analogy is a perfect one, where the human race has up and down cycles but always trends up longer term thanks to technological innovation.
My one criticism is he speaks out of both sides of his mouth in one instance, presumably because he doesn’t want to upset any high ranking politicians or leaders he may be friends with, which I found to be disappointing.
On the one hand, he notes at the start of the book that no two democracies have waged war with each other, wars have only been fought between dictators/police states and democracies or just dictators/police states.
Then later on when discussing China, he all but excuses and rationalizes their increasingly authoritarian state, as seen by Xi crowning himself leader until death and abolishing the precedent of 2 5 year term limits as of 2018. He blesses the Chinese approach of a few rulers knowing what’s best for all, as if those rulers are acting in the broader interest of Chinese people, and that’s an acceptable alternative to democratic rule. He cites the recent video game ban as having merit or at least being understandable, suggesting that he thinks the ends can justify the means. All the while there’s no mention of the atrocities of Mao under this authoritarian type of rule, no mention of the Muslim genocide going on now, the suppression of free speech and jailings and beating and murders of those that oppose the current regime, no mention of internet censorship, etc.
To bring the criticism full circle, he doesn’t link his first point on wars and authoritarians always being involved in them, with the fact that China is an authoritarian state and therefore it’s rise threatens the free world and human progress. Ironically, he does correctly acknowledge China’s opening up to market and establishment of capitalist principles for rocketing them toward the US in terms of power, while refuses to critique the political system despite its history of failings, violence and pain. Russia invading Ukraine couldn’t drive this point (ie the civil or political system being as important as the economic system to the long term success of a country and world peace) home any harder.
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Reviewed in the United States on March 12, 2022
★★★★★ 5
Can be effectively used as a working tool for the predictor (not just for investments)
Format: Hardcover
This is one of the best works on the declining economy and US, associated possible revolution/civil war and later major power war, that is presented in a clear, convincing and replicable way. Kudos to Dalio!! More importantly, the contents of the book can be used to predict upcoming events rather than just perceiving the world on fire with several likely upcoming breakouts (e.g., war with China over Taiwan, the likely loss of our reserve currency, the unsustainable and uncontrollable burgeoning national debt which grows by a trillion USD every 100 days). The author makes a valid case that significant events are moving very rapidly and, for the rest of the 2020s, things are going to get a whole lot worse for the non-elites in our society. Of note, the book was actually written in 2020, published the next year, which then allows the readership now in 2025 to verify the accuracy the observations and predictions. Right on target!
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Reviewed in the United States on June 4, 2025
★★★★★ 5
Great book for understanding how the world works!
Format: Hardcover, Format: Hardcover
Dalio has a unique perspective on the topic of the changing world order. He is a successful businessman who has spent his career analyzing economic trends and patterns, and this book is a culmination of his findings. His writing is clear and concise, making complex economic concepts easy to understand.
One of the best features of the book is its organization. The book is broken down into 14 chapters, each focusing on a different aspect of the changing world order. Dalio starts with the big picture, examining the major forces driving the changing world order, before delving into the specifics of each major empire, including the Dutch, British, American, Chinese, Soviet, and Japanese empires. Ultimately, he brings everything full circle by discussing the changing world order today and what the future may hold.
Another great aspect of the book is the way that Dalio weaves history and economics together. He doesn't just present economic theories in a vacuum; he uses real-world examples to show how they have played out over time. For example, in Chapter 5, he discusses the Great Depression and how it shaped the changing world order in the 1930s and 1940s. He also uses the rise of populism in Chapter 7 to illustrate how economic inequality can lead to political instability.
Overall, I would highly recommend "The Changing World Order" to any intelligent human interested in economics, history, or politics. This book is a must-read for anyone who wants to understand the forces shaping our world today and what the future may hold. As Dalio himself puts it, "understanding how the world works is essential if you want to accomplish your goals and live a fulfilling life."
Here are some key takeaways from the book:
The changing world order is driven by three major forces: the changing relative powers of countries, the changing relative productivity of countries, and the changing values of countries.
The rise and fall of empires is a natural part of the changing world order. Each empire has its own unique characteristics, but they all follow a similar pattern of rise, peak, and decline.
The post-World War II order was built on the idea of free trade and cooperation between nations. However, this order is now under threat due to rising nationalism and protectionism.
China is currently on the rise and is likely to become the world's dominant economic power in the coming decades. However, although this rise is not guaranteed, and there are many challenges that China will need to overcome, the US needs to step up its game on several fronts to compete.
The future of the world order is uncertain, but there are a few things we can say with some degree of certainty. For example, the rise of automation and artificial intelligence is a hot topic today likely to have a major impact on the global economy in the coming years.
Overall, "The Changing World Order" is a well-written and informative book that is sure to appeal to a wide range of readers. Whether you're a history buff, an economics nerd, or just someone who wants to better understand the world we live in, this book is well worth your time. As Dalio himself says, "The more you know, the more you'll understand, and the more you'll be able to make informed decisions about your own life." Five stars from me, give it a read!
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Reviewed in the United States on May 9, 2023
★★★★★ 5
10/10 Recommend
Format: Hardcover
I took Jeffrey Sachs's Globalization class on EDX Academy and somehow stumbled upon this book in between class and finding books around the subject.
I watched his youtube video that generalizes the book and was blown away by how my current class I was taking aligned with it.
This book is an easy read, and especially for those who aren't well-versed about world history and world economics. I will admit that I do love history, and am learning economics, so this book was a beautiful way merge all these timelines together.
The book breaks down and summarizes key points in world history and economics to make points to get the message across each chapter.
Font size is great! Might even be considered larger compared to other books.
The only thing I wish this book provided was thicker paper in the physical book itself, especially for the hardcover version.
If Ray Dahlio ever comes out with a special edition of this with a higher quality paper, I would gladly purchase it for my collection.
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Reviewed in the United States on March 2, 2025
★★★★★ 5
Excellent book – exciting and educational!
Format: Paperback
This book is a true gem! It took us on a journey back in time and revealed the real story of the Olympic Games in a simple, vivid, and perfectly understandable way for children. My young students loved it, and I found it extremely useful and well-written. Ideal for kids who love history, sports, or simply want to learn something different in a fun way.
Highly recommended!
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Reviewed in the United States on June 9, 2025