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Description
Mouse TL ELISA KitProduct Specification Sensitivity 9. 375ng ml Species Reactivity Mouse Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin labeled anti TC antibody. Free components are washed away. HRP streptavidin (SABC) is
Product Specification
| Sensitivity | 9.375ng/ml | ||||||||||||||||||||||||||||||||||
| Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||
| Theory | This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre-coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin-labeled anti-TC antibody. Free components are washed away. HRP-streptavidin (SABC) is added, and biotin specifically binds to streptavidin to form an immune complex. The unbound conjugate was washed off and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP) and turned yellow after the reaction stop solution was added. The OD value was measured at 450nm with a microplate reader. Using curve equation software, the OD450 value of the sample was compared to the standard curve to determine the concentration of TC in the sample. There is an inverse proportion between the concentration of the target substance and the OD450 value. | ||||||||||||||||||||||||||||||||||
| Synonym | Mouse TC (Total cholesterol) ELISA Kit | ||||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | ||||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure. 2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture). 3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation. 4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds. 5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure. 6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation. 7. Do not reuse disposable tips to avoid cross-contamination. 8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature. 9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations. |
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| Instructions | Serum, plasma, cell culture supernatant, cell lysate and other fluid samples | ||||||||||||||||||||||||||||||||||
| Storage Temp. | Unopened kit, stored at 2-8 °C, shelf life for one year. | ||||||||||||||||||||||||||||||||||
| Test Range | 15.625-1000ng/ml |
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4.6 ★★★★★
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Product Reviews
★★★★★ 5
Great product
Highly recommend for teething. - different texture keeps puppy amused- little pricy - actually was cheaper in local store but already bought here.
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Reviewed in the United States on April 29, 2020
★★★★★ 5
Dog loves it- lasts long
Much bigger than expected
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Reviewed in the United States on November 20, 2020
★★★★★ 5
Lasts a long time
Very durable and my dog loves it. He’s not a big guy but is a determined chewed. Either he destroys the toys in a day or two or has not interest in chewing it. This keeps his interest and I has lasted a week with only a little wear to it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 24, 2020
★★★★★ 5
Highest rated chew toy in my house! Worth it!
Pro’s - I have mini Daschund’s and a 50lb Aussie and these are the favorite chew toys in the house. We have 4 dogs total and at first there was only one and all others would stare at the one that had it till he got board then it was war. Because of this I bought more.
Con’s - they are Hard plastic and are loud when they are playing with them on hardwoods, like super loud. Also they are not forgiving if stepped on.
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Reviewed in the United States on January 13, 2021
★★★★★ 5
My super chewer loves this toy!
This toy is a hit for our 1 year old puppy. She’s a super chewer and destroys every toy we give her within days. The toys has lasted a month and keeps her occupied for hours!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 27, 2022
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