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Description
Rat PER2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Cell Supernatant: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse the tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Preparation before testing: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the gradient working solution of the standard: Add 1 mL of universal diluent to the lyophilized standard. Let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Period Circadian Protein 2 (PER2) capture antibody. After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Period Circadian Protein 2 (PER2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Period Circadian Protein 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | PER2 is a protein encoded by the PER2 gene in mammals. PER2 has attracted attention for its central role in circadian rhythms. Disorders associated with PER2 include advanced sleep phase syndrome, familial 1, and advanced sleep phase syndrome. Pathways associated with PER2 include the metabolism and effects of the circadian clock and melatonin. Gene ontology annotations associated with this gene include transcription factor binding and transcription cofactor activity. An important homologue of this gene is PER1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell supernatant, tissue homogenate, etc. |
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4.4 ★★★★★
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Product Reviews
★★★★★ 5
Very well made, matches width of Keychron Q6 perfectly, great for bigger hands!
This is a nicely finished slab of wood that is an EXACT match for my Keychron Q6 in both width and height. Depthwise, this is 4", so if you got larger hands (as I do), this is a bit better than the silicone Keychron version which I believe is 3.5" or so in depth. The non-stick feet are a nice feature, and the profile is wonderful to type on. Only issue with the item I received is a small nick right below the Glorious emblem on the lower right of the wrist rest - may have been an errant tool mark. However, this doesn't detract in my own opinion, from the well-designed profile and features of this wrist rest and was easily covered with a furniture marking pen. I'm still giving this item 5-stars and getting another one for the office!
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Reviewed in the United States on May 7, 2025
★★★★★ 4
Glorious Wooden Palm Rest Durability and Stability in an Elegant Design
Color: Onyx, Size: Mouse Wrist Rest, Color: Onyx, Size: Mouse Wrist Rest
After years of using foam palm rests, I always encountered the same problem: they deformed over time, losing height, ergonomics and, consequently, their usefulness. This is especially critical when you spend between 3 and 8 hours a day at the PC for work, study or gaming, since a bad posture can increase the risk of suffering from carpal tunnel syndrome.
This is where Glorious' wooden palm rests come in, a solution that eliminates deformations and offers an ideal height for both the mouse version and the TKL (Tenkeyless) version.
Positive points
• Solid and durable construction: Unlike foam, wood maintains its shape and provides stable support over time.
• Elegant and minimalist design: Its finish gives a premium touch to any setup.
• Effective non-slip base: The bottom rubbers prevent unwanted movements, providing total stability.
• Easy maintenance: Although wood is slightly porous and can retain natural oils or residue from creams and perfumes, cleaning it is easy.
Areas for improvement
• Less softness than foam: Although still comfortable, wood sacrifices some of the cushioned feel that some prefer. A redesign with a foam strip on top could improve this.
• Inconsistent finish between versions: I bought the mouse rest and the TKL at the same time, but noticed that the color tones were different (one blacker than the other). It doesn't affect functionality, but it does affect aesthetics.
If you're looking for durability, stability, and a sophisticated design, this is a highly recommended palm rest, especially if you find it on sale. Although it's not as soft as the foam ones, it does its job of providing ergonomic support exceptionally well. An excellent complement for those who spend long hours at the PC and are looking for a sturdier alternative to traditional palm rests.
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Reviewed in the United States on February 5, 2025
★★★★★ 5
great mouse rest
Color: Golden Oak, Size: Mouse Wrist Rest
This works really well for for elevating your wrist, I use an apple trackpad it puts my wrist a little too high so what I did was put some rubber pads on the back of my trackpad to raise it up. It now gives the perfect height and good support I can even lean on it. It works well to the fact that it gives you very good support it's not uncomfortable and it's very easy to clean and looks very nice on my desk, because it's made out of real wood.
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Reviewed in the United States on March 13, 2022
★★★★★ 5
Great wrist rest. Smooth finish, good looks, and ideal wrist angle.
This wrist rest sets you up for pretty optimal, level height to prevent future wrist problems. I went with the nice reddish/brown color as it matches my brown wood Edifier speakers and other brown accessories very nicely. Nice matte finish that avoids showing finger prints and skin oils. The color is perfect, not too red or too brown.
Keep in mind if your keyboard is angled in the front it will leave a bit of a gap, however it is unnoticeable to me. Also, make sure your arm rests of your chair can come high enough to meet the half inch or so thick wrist rest. My arm rests are a bit low for the time being so some extra pressure is put on my arm behind the wrist when resting on the chair supports.
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Reviewed in the United States on March 29, 2021
★★★★★ 3
Not for me
Color: Golden Oak, Size: Mouse Wrist Rest
It's tough to review something like this that functions perfectly, but just wasn't the right fit. The Glorious wrist rest for the keyboard is perfect and exactly what I wanted/needed, but the mouse rest caused hand and wrist pain. I swapped it out for a squishy alternative and that worked much better. I have a theory it might have been due to the relatively low height of my ektecity gaming mouse relative to the mouse rest. My keyboard is a higher height and that may be why the wood keyboard rest works so much better.
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Reviewed in the United States on April 22, 2024