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Description
Human TMX2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a thioredoxin-related transmembrane protein 2 (TMX2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of thioredoxin-related transmembrane protein 2 (TMX2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Thioredoxin-related transmembrane protein 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Thioredoxin-related transmembrane protein 2 (TMX2) is encoded by the TMX2 gene, a member of the endoplasmic reticulum (ER) protein disulfide isomerase (PDI) family. This protein catalyzes protein folding and thiol-disulfide exchange reactions. The encoded protein has an N-terminal ER signal sequence, a catalytically active thioredoxin domain, a transmembrane domain, and a C-terminal ER retention sequence. This protein is enriched in mitochondrial-associated membranes of the ER through palmitoylation of two membrane-exposed cysteines. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.9 ★★★★★
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Product Reviews
★★★★★ 3
Good Stability, But Quality Could Improve
I bought this bathroom shelf on Amazon and overall, I’m fairly satisfied with the purchase. It is sturdy and holds my items securely without wobbling. The assembly process was straightforward and didn’t take much time. However, I did expect it to be a bit wider based on the pictures, so make sure to double-check the measurements before ordering. I also wish the overall quality of the materials was a little better, as some parts feel slightly less durable than I expected. That said, it still does the job well and serves its purpose. It’s not a bad purchase, especially if you’re looking for something functional and affordable.
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Reviewed in the United States on February 24, 2026
★★★★★ 5
Sturdy and good looking.
Size: 24x5.8, Color: Black, Size: 24x5.8, Color: Black
Great shelves. As you’d expect - and mounting is just fine if you pay attention and hit some studs. I’ve got my wife’s plants on these for over a year with no problems.
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Reviewed in the United States on October 26, 2025
★★★★★ 5
great shelves
Color: C. White - Modern Minimalist Style, Number Of Shelves: 3, Size: 36inches
These are a a great set of floating shelves! They were easy to install, feel sturdy, and look clean and modern on the wall. The set of three is perfect for displaying decor, photos, plants or small storage items while adding extra space without taking up room. Great quality, stylish, and a simple way to upgrade any space. They work perfect for my figurines to be displayed.
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Reviewed in the United States on May 19, 2026
★★★★★ 5
Great shelves
Color: A. Black - Universal Classic Style, Number Of Shelves: 3, Size: 22.5inches
Easy to hang and look great in my office. I love the little divot along the edge. Helps to keep pictures from sliding.
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Reviewed in the United States on May 29, 2026
★★★★★ 5
Exactly what I needed for my bathroom refresh.
Color: B. Rustic Brown - Americana Farmhouse Style, Number Of Shelves: 2, Size: 22.5inches, Color: B. Rustic Brown - Americana Farmhouse Style, Number Of Shelves: 2, Size: 22.5inches
I bought these floating shelves in the color Rustic Brown to replace a bulky storage shelf that was making my bathroom feel cramped, and they completely transformed the space. The wood finish looks much more expensive than I expected, and once installed they gave the bathroom a clean, modern, spa-like feel without taking up visual space.
Installation was straightforward overall. I mounted one screw in the center into a stud and used drywall anchors for the remaining hardware, and the shelves feel very sturdy. They sit flush against the wall and don’t sag or wobble. I especially love how slim they are while still being deep enough to hold everyday bathroom items, decor, candles, skincare, and small baskets.
The color worked perfectly with my bathroom vanity and mirror trim, and the floating design instantly made the room look more organized and intentional. Huge upgrade for a small space.
Definitely recommend if you want functional storage that also looks elevated and minimalist.
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Reviewed in the United States on May 30, 2026
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