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Description
Human PML ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a promyelocytic leukemia protein (PML) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of promyelocytic leukemia protein (PML) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Promyelocytic Leukemia Protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Promyelocytic leukemia protein (PML), also known as MYL, RNF71, PP8675, or TRIM19, is the protein product of the PML gene. This protein is a tumor suppressor required for the assembly of numerous nuclear structures, called PML nuclear bodies, which form within the chromatin of the cell nucleus. These nuclear bodies are present in mammalian cell nuclei, with approximately 1 to 30 per nucleus. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.1 ★★★★★
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Product Reviews
★★★★★ 5
Great Clean Alternative to Traditional Mouthwash, LOVE this!!
Flavor Name: Mint, Size: 3.5 Ounce (Pack of 1)
I’ve been really impressed with these Boka mouthwash tablets. They’re fluoride‑free and alcohol‑free, which is exactly what I was looking for, and the hydroxyapatite is a nice bonus for supporting enamel without the harshness of traditional mouthwashes. The tablets dissolve easily, the flavor is refreshing without being overpowering, and there’s no burning sensation at all. Do NOT chew and swallow like I did the first time. Chew, get salva going and swish. My teeth never felt so clean!!!
I also love that they’re travel‑friendly and cut down on plastic waste compared to big bottles. My breath feels fresh, and my mouth feels clean without that chemical aftertaste. If you’re trying to switch to a cleaner, gentler oral‑care routine, these are a great option.
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Reviewed in the United States on March 25, 2026
★★★★★ 5
Tasty, effective, and helpful against thrush
Flavor Name: Citrus Mint, Size: 3.5 Ounce (Pack of 1)
This is a good plastic-free, portable form of mouthwash. Pop one or two tablets in your mouth, chew well, add a little water if your mouth is particularly dry (mine is), swish, and spit. That's it. They don't burn like regular mouthwash, and they leave a nice flavor that isn't overpowering and that lasts longer than other mouthwashes have for me. It's a balanced flavor, if that means anything to you - basically, my mouth somehow feels right afterwards.
I was skeptical about the claim that these improve the mouth microbiome, but they've virtually eliminated the thick white layer (presumably thrush) that I've been fighting to clear off my tongue forever. So, yeah, these are definitely a winner.
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Reviewed in the United States on December 24, 2025
★★★★★ 4
More like a mint candy than mouthwash.
Flavor Name: Mint, Size: 3.5 Ounce (Pack of 1)
Hard to describe this product. Does it work like your typical mouthwash? No. Does it leave your mouth feeling minty and fresh? Yes, to a certain extent. Would I consider this an alternative to mouthwash on a consistent basis? No, it's ok for traveling because it's easy to transport.
When you chew this tablet, it has the texture and feel of a traditional mint. It's chalky and breaks up easily.
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Reviewed in the United States on April 28, 2026
★★★★★ 5
Great Product
Flavor Name: Citrus Mint, Size: 3.5 Ounce (Pack of 1)
I absolutely love this product! It keeps my breath fresh and no sensitivity issues.
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Reviewed in the United States on May 30, 2026
★★★★★ 3
Not a fan of this one
Flavor Name: Mint, Size: 3.5 Ounce (Pack of 1)
I am not a fan of this product. As many of the reviews noted, the flavor is very strong. I was surprised it wasn't more minty. This is a very herbal concoction. It also tastes of the other very strong herbs, especially the rosemary. According to the instructions, this product should be used once a day for 30 seconds. I prefer a product I can use twice a day for 45 seconds to a minute.
My experience is that it takes 30 seconds just to crush it up enough to swish it around and I don't have enough saliva in the morning due to overnight dry mouth. I have to add a little water. The tablets never crush completely and leave a mess in the sink that has to be carefully rinsed away. If I use them more often, my mouth dries out too much and tastes like the tablets all day long. It's not a bad taste but I expect it to go away after a half-hour (or so). They are really chalky.
The container isn't bad, but doesn't pour 1 tablet out at a time. Meh. I don't like touching a bunch of them each time. There is a desiccant packet in with the tablets to protect them. I am not sure this would stay closed during travel or be allowed on a plane in carry-on. It should travel ok otherwise because it's not a liquid, but I haven't tried it. I will probably not buy this product again, but in all honesty, I have bought far worse products and been disappointed far more by them.
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Reviewed in the United States on December 3, 2023