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Description
CHAF1b Recombinant Rabbit mAb (S-R316)Product Specification Host Rabbit Antigen CHAF1b Synonyms Chromatin assembly factor 1 subunit B, CAF 1 subunit B, Chromatin assembly factor I p60 subunit (CAF I 60 kDa subunit; CAF I p60); M phase phosphoprotein 7, CAF1A, CAF1P60, MPHOSPH7, MPP7 Location Cytoplasm, Nucleus Accession Q13112 Clone Number S R316 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ICC, ICFCM, IP Reactivity Hu Purification Protein A Concentration 0. 5 mg ml
Product Specification
| Host | Rabbit |
| Antigen | CHAF1b |
| Synonyms | Chromatin assembly factor 1 subunit B, CAF-1 subunit B, Chromatin assembly factor I p60 subunit (CAF-I 60 kDa subunit; CAF-I p60); M-phase phosphoprotein 7, CAF1A, CAF1P60, MPHOSPH7, MPP7 |
| Location | Cytoplasm, Nucleus |
| Accession | Q13112 |
| Clone Number | S-R316 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, ICFCM, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IHC-P | 1:500 | null |
| ICC | 1:500 | null |
| ICFCM | 1:500 | null |
| IP | 1:50 | null |
Background
Chromatin assembly factor I (CAF-1) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. CHAF1b corresponds to the p60 subunit and is required for chromatin assembly after replication. It is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
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