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Description
Human SMOC2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a SPARC-related modular calcium-binding protein 2 (SMOC2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of SPARC-related modular calcium-binding protein 2 (SMOC2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader, and the sample concentration is calculated. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human SPARC-related modular calcium-binding protein 2ELISA kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | SPARC-related modular calcium-binding protein 2 (SMOC2) is a protein encoded by the SMOC2 gene. This gene has been shown to be mutated in clinical cases of major tooth developmental defects. Disorders associated with SMOC2 include dentinogenesis imperfecta type I and dentinogenesis imperfecta type II. Pathways associated with SMOC2 include: Gastric Cancer Network 1. Gene Ontology (GO) annotations associated with this gene include calcium binding and glycosaminoglycan binding. An important homolog of this gene is SMOC1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.4 ★★★★★
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Product Reviews
★★★★★ 5
Fast shipping
Color: Blue, Memory Storage Capacity: 128 GB, Set name: Wi-Fi + Cellular
Item as described, mint condition, works great came with charger block and fast shipping only issue I had is with shipping carrier. FEDEX stinks showed arrival as Monday, was delivered 1/21 10:45 am yet their system showed package was in Louisville, Tn at this time later in the evening I checked again showed delivered. No package by my door from FedEx had to go to their website to find picture where the left it. Was found at entrance to my complex on top of mailboxes where anyone could have of taken it…
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Reviewed in the United States on January 22, 2026
★★★★★ 5
Very satisfied with purchase!
Color: Silver, Memory Storage Capacity: 256 GB, Set name: Wi-Fi + Cellular
Everything worked as advertised. Nice 11rh generation iPAD.
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Reviewed in the United States on April 30, 2026
★★★★★ 5
Perfect condition
Color: Silver, Memory Storage Capacity: 512 GB, Set name: Wi-Fi + Cellular, Color: Silver, Memory Storage Capacity: 512 GB, Set name: Wi-Fi + Cellular
Works perfectly fine, no scratches or dents. Battery life is great. Worth the purchase.
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Reviewed in the United States on April 14, 2026
★★★★★ 5
Just Love this.... Read On!
Color: Silver, Memory Storage Capacity: 128 GB, Set name: Wi-Fi + Cellular
Like knew and seems to have been powered on one time only. Apple treat it as a new device.
Its spotless and works great.
I have had several ipad pro's and decided to by this instead and I actually prefer it. It does everything I could do with my ipad pro 2020 and then some. Its faster, good cameras. The e-sim card works great though I do wish it had a physical sim card slow.. oh well!
The battery life is really excellent, it was quick to set up via a transfer from my old device. I added a case that looks like a fancy note book and love it. This helps with drops and scratches. I have not added a screen protector yet.
The storage capacity is the same as my old ipad pro, I will never use 128 gb anyways...
To sum up, this gets 10 out of 10... and its a great replacement for a ipad pro!
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Reviewed in the United States on November 13, 2025
★★★★★ 5
Great conditions just like new
Color: Yellow, Memory Storage Capacity: 128 GB, Set name: Wi-Fi + Cellular
Hey I usually don’t leave reviews but I do feel
Like this one needed one thank you for sending out the iPad well packed .. I received it in great condition it’s been working pretty good no complains I got it for my 3 year old son who’s on his third iPad lol hopefully this is his last , just like new I love it no scratches nothing literally brand new thank you
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Reviewed in the United States on May 24, 2026